18S rDNA PCR


Description

Accurate identification of fungal species, particularly Candida, is essential for the interpretation of anti-fungal sensitivity testing (MICs) and successful treatment. Identification of fungi based on phenotypic and morphological characteristics can be both difficult and slow. Classification of fungi by their ribosomal gene sequences is now widely accepted. PCR amplification of 18S ribosomal gene sequences followed by DNA sequencing and comparison to ribosomal sequence databases allows the classification of most Candida species and many other fungi. This broad-range fungal PCR can also be used to detect a wide range of fungal species in primary clinical specimens.

Also known as

18S PCR, fungal PCR, broad-range fungal PCR, pan-fungal PCR, ribosomal PCR


Request a test

To request this test please send sample with a request providing patient ID (three identifiers), specimen information, assay required, relevant clinical details and sender information. Before sending sample please read details on requesting and labelling by clicking on the link. Please also refer to any additional information provided for this test. Download the referral form >


Additional information

Not applicable


Sending address

Microbiology, Virology and Infection Control Level 4, Camelia Botnar Laboratories Great Ormond Street Hospital for Children Great Ormond Street London WC1N 3JH DX number: DX6640203 DX Exchange: Bloomsbury 91WC

Laboratory service

Microbiology, Virology and Infection Control


Sample requirements

Any sample from a normally sterile site. Minimum volume 200 microlitres. Isolates on slopes.


Reference range

Not applicable


Turnaround time

48 hours - 7 days


Disease / group

Fungal infection


Cost

Upon request


Call in advance?

Not required


Microbiology, Virology and Infection Control

Our laboratory performs a wide range of routine and specialist investigations in Bacteriology, Virology and PCR.

© 2011 Great Ormond Street Hospital for Children NHS Trust