EWS-ERG


Description

Translocation Detection by Reverse transcriptase polymerase chain reaction (RT-PCR). All tests are designed to amplify PCR products less than 200bp. Tested in conjunction with EWS-FLI1. All tests are comlemented by amplification of a relevant housekeeping gene to test the quality of the RNA.


Request a test

When requesting this test please use the referral form provided. Please also refer to any additional information provided for this test. Download the referral form >


Additional information

Ewings sarcoma/ Primitive neuroectodermal tumour (PNET) is the second most common bone and soft tissue in children. It is characterised by an EWS-FLI1 fusion gene which is detected in approximately 85% of cases. Approximately 10-15% of cases have a variant EWS-ERG fusion gene.


Sending address

Paediatric Malignancy Unit Great Ormond Street Hospital for Sick Children Level 2, Camelia Botnar Laboratories Great Ormond Street London WC1N 3JH

Laboratory service

Paediatric Malignancy Unit


Sample requirements

Fresh, frozen or FFPE tissue. If sending FFPE rolled tissue sections please send the equivalent of 80 uM of tissue.


Reference range

Not applicable


Turnaround time

3-5 working days


Disease / group

Ewings Sarcoma


Cost

Upon request


Call in advance?

Please call in advance to discuss requirements


Paediatric Malignancy Unit

The Paediatric Malignancy Unit is a unit providing a wide range of molecular genetic and cytogenetic diagnostic tests.

© 2011 Great Ormond Street Hospital for Children NHS Trust